Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation AssayEfstathia Thymiakou 1, Vasso Episkopou 1
1Medical Research Council, Clinical Sciences Centre, Imperial College, Hammersmith Hospital
Here we show how to use Proximity Ligation Assay (PLA), with a combination of antibodies to visualize Bone Morphogenetic Protein (BMP) signaling in fixed cells. This technique allowed us to follow the nuclear accumulation of endogenous BMP activated effector-complexes and quantify their levels over time under BMP4 stimulation.
